dihydroxyacetone phosphate to pyruvate

0000002150 00000 n ���Iʕ+H���B���r�!o�P2;⎢l�xG9I�C���pD��0����S4K6챵����ы�A���!���,���w���E�X�����s��5��*�ڴnV��0��mϨg�P"Y�~�1+C��� dU��І�RE�(.B}�a���c�O4e. glyceraldehyde-3-phosphate and dihydroxyacetone phosphate. Pyruvate in animals can also be reduced to lactate by adding electrons from NADH (Figure 6.24). 385 0 obj <> endobj 0000006174 00000 n Blood was stored with various combinations of 60 mM dihydroxyacetone (DHA), 80 mM pyruvate and 10 mM phosphate in citrate-phosphate-dextrose-adenine-one (CPDA-1) preservative for 42 days. 0000002533 00000 n Reaction 8/10: 3-phosphoglycerate to. 0000005125 00000 n This enzyme was immediately inactivated by exposure to the air. 0000046849 00000 n Reaction 7/10: 1,3-bisphospho=glycerate to. 0000020309 00000 n 1,3-bisphospho-glycerate. ��.uF! 0000007990 00000 n Dihydroxyacetone, pyruvate, and phosphate effects on 2,3 DPG and ATP in citrate-phosphate-dextrose-adenine blood preservation. Enzyme activity was unstable even when stored ana … Thus, it is isomerized into glyceraldehyde 3-phosphate instead. The conversion of fructose 1,6-bisphosphate to fructose 6-phosphate is not catalyzed by phosphofructokinase-1, the enzyme involved in glycolysis. 0000005345 00000 n Although this enzyme is found in mitochondria, peroxisomes appear to be a more important location. J Biol Chem. 0000005848 00000 n Write balanced biochemical equations for all the reactions in this process, with the standard free-energy change for each reaction. The methylglyoxal pathway is activated by the increased intercellular uptake of carbon containing molecules such as glucose, glucose-6-phosphate, lactate, or glycerol.Methylglyoxal is formed from dihydroxyacetone phosphate (DHAP) by the enzyme methylglyoxal synthase, giving off a phosphate group.. Methylglyoxal is then converted into two different products, either D-lactate, and L-lactate. Formation of a Schiff base (imine) with an active site lysine followed by conversion to the enamine produces the nucleophilic species. Dihydroxyacetone phosphate (DHAP, also glycerone phosphate in older texts) is the anion with the formula HOCH2C(O)CH2OPO3 . Pyruvate is a “starting” point for gluconeogenesis, being converted to oxaloacetate in the mitochondrion in the first step. 0000030291 00000 n ��Z�gm���%��=������t����;�~g� 0�Q���G�z]����q������RR(���@�ڦ>���쇓����~O�Ły�r����p 1969 Apr 25; 244 (8):2075–2077. 0000002207 00000 n � C�ל�\���.��v��X�Hm=s+ ���>zƆ)i���5��D配����h���*����’�)������,�x�l�Z�����@���X��0 0000036554 00000 n Schematic representation of engineering strategies for FFA production in S. cerevisiae. �@޽>9�)��4,R�Y+ ⒙�j��4-/����@r�:!��fB�d����� h������((l���,. Reaction 4/10: fructose-1,6-phosphate to. How is glycolysis maintained under anaerobic conditions? Dawson RB, Fagan DS, Meyer DR. 0000003072 00000 n Le cycle de Krebs 30 5. 438 0 obj<>stream 0000046134 00000 n 0000045124 00000 n <<101BAB085D6FCE4899921BA5B0E13529>]>> Un article de Wikipédia, l'encyclopédie libre. glyceraldehyde-3-phosphate. Entry field with correct answer 1 mol of NADH + 1 mol of ATP 2 mols of NADH + 4 mols of ATP 1 mol of NADH + 2 mols of ATP 1 mol of NADH + 4 mols of ATP 2 mols of NADH + 2 mols of ATP. 7@ (������s�O�$���L&j#��)%�ϯ)�V��o�� ��{��[��0 ��#��L Pt�Ux��SP*b%���"� U�E4:�p*{XTLb�Uq���^ޱ�K�Ec�*������C�'c㙴d�]�K��%[ ���m�N(���%>9��K��� %%EOF Methyl pyruvate (MPA) was synthesized from dihydroxyacetone (DHA), the oxidation product of glycerol, over Sn-β and TS-1 hybrid molecular sieves at low temperature. C'est avant tout un métabolite de la glycolyse, mais il joue également un rôle dans le cycle de Calvin et dans la biosynthèse de certains lipides de Leishmania mexicana [réf. This anion is involved in many metabolic pathways, including the Calvin cycle in plants and glycolysis. b) When dihydroxyacetone phosphate is directly converted to 1,3-bisphosphoglycerate the reaction rate is too rapid. 0000046419 00000 n 0000002035 00000 n Kerr HH, Pantely GA, Metcalfe J, Welch JE. What are the primary metabolic fates of pyruvate? The regeneration of _____ in the reduction of pyruvate to lactate sustains glycolysis under anaerobic conditions. 0000012819 00000 n phosphophoenol-pyruvate. 5. 0000001376 00000 n 0000011386 00000 n UeyQT��o��5"B����k�� !��]W�>���j���钗��Fq���tm�u�������7;C��T���a6vs*���Kcݝ�=� i&Y���KZ:l����s�s��,��ʐ���S�ϱZ�y*C6�Ş5��ٹ$�h�����n��_���[;�5gC߹�>��4L�h���D�t$+��CP�7�,K[fE%k� �56P����֯Sp�6\R\��6�Q����.��W�&V��v��u�|�Lk�3Q��F���Z����h;۠G�-��$�� �R��o�~��/h�4e���|�1��y�Ӑ38�h���(�.�Q�������a1 �d�0@�"/���E`�#�#�}pSKQ4P��nHs�����)���zE��)�Uz߆Ͷ�N����/A]VwnQ�z��S Glycerol-3-phosphate dehydrogenase (GPDH) is an enzyme that catalyzes the reversible redox conversion of dihydroxyacetone phosphate (a.k.a. reduction to lactate. 0000009401 00000 n The fourth step in glycolysis employs an enzyme, aldolase, to cleave 1,6-bisphosphate into two three-carbon isomers: dihydroxyacetone-phosphate and glyceraldehyde-3-phosphate. 0000047885 00000 n What is the net energy gain when 1 molecule of dihydroxyacetone phosphate is converted to pyruvate? Recently, [2- 13C]dihydroxyacetone (DHA) has been demonstrated for hyperpolarized imaging of the liver (Moreno et al., 2014). 0000002641 00000 n G3P glyceraldehyde-3-phosphate, 3PG 3-phospho-glycerate, cPDH cytosolic pyruvate dehydrogenase. nécessaire]. Reduction of human blood O2 affinity using dihydroxyacetone, phosphate, and pyruvate. 0000046657 00000 n A cytosolic PDH complex was evaluated in a faa1Δ faa4Δ pox1Δ hfd1Δ mutant YJZ08 for free fatty acid production, in combination with fatty acid metabolism engineering via pah1Δ lpp1Δ dpp1Δ are1Δ and … DHA has many qualities similar to pyruvate that suggest it may be successfully used as an HP imaging agent. 0000004816 00000 n Human blood oxygen affinity (BOA) was measured after blood from 6 normal donors was incubated with 4 concentrations of dihydroxyacetone (0.022, 0.044, 0.088, and 0.175 M) plus equimolar disodium phosphate and pyruvate (sodium salt) (0.013, 0.025, 0.05 and 0.1 M) in solutions labeled DDP x 1, DDP x 2, DDP x 4, and DDP x 8, respectively. L'ion pyruvate est le produit final des voies de dégradation du glucose (la glycolyse, voie des pentoses phosphates, voie d'Entner-Doudoroff). 0000002856 00000 n Ainsi, chaque molécule de β-D-fructose-1,6-bisphosphate donne en fin de compte deux molécules de G3P. 0000011559 00000 n 1. Activation of a fatty acid prior to its entry into the fatty acid spiral requires the presence of (Points : 1) O FAD O malonyl CoA O cyclic AMP O ATP 3. ethanol lactate acetyl-CoA. startxref !�\�l+�l���b��~�Z�~���Żt�+��s�"�J�1�l8�$��Lk^"� {�ڃk�. Le métabolisme du pyruvate 29 3. are pyruvate, phosphoenol pyruvate, acetaldehyde, glycine, and dihydroxyacetone phosphate (DHAP), giving rise to the synthetically most useful classification of aldolases (Fessner and Walter 1996). 0000046474 00000 n Step 5. 0000003178 00000 n 0000043842 00000 n Conversely, triglycerides can be broken down into fatty acids and glycerol; the latter, in turn, can be converted into dihydroxyacetone phosphate, which can enter glycolysis after the second control point. In glycolysis dihydroxyacetone phosphate isomerizes to glyceraldehyde 3-phosphate because: a) When dihydroxyacetone phosphate is directly converted to 1,3-bisphosphoglycerate the reaction of too exothermic. The Payoff Phase of Glycolysis in Skeletal Muscle In working skeletal muscle under anaerobic conditions, glyceraldehyde 3 -phosphate is converted to pyruvate (the payoff phase of glycolysis), and the pyruvate is reduced to lactate. The dihydroxyacetone phosphate can be removed from glycolysis by conversion into glycerol-3-phosphate, which can be used to form triglycerides. 0000035846 00000 n trailer x�b```��l DAG activates protein kinase A Le shunt glyoxylique 31 6. The conversion of glucose 6-phosphate to glucose is catalyzed by hexokinase, the same enzyme involved in glycolysis. 0000010116 00000 n Pyruvate-dihydroxyacetone supplementation has also been found to improve the insulin sensitivity of insulin resistant rats and reduce plasma cholesterol levels induced by a high cholesterol diet as well as lower blood pressure and heart rate in obese individuals. ]�Lʒ�TF�"F�~��xIA��~%x� /N����T�J When infused in rats during prolonged treadmill running, pyruvate reduced run time to exhaustion by approximately 67%. @�a3i���T�:��0�T���q(�%Q�`ll���р;,��8��� �@Qa�cRb�`�aibpcfЙ0ǡ����e�^�֙�5�.-��T|�jE�20����]�|hA���h���?|8 ��d��L.�4)qf�r� ��&2���ؠ�i�E����(�������H�8L|\�$��*��50(2�v}��@�(����C��c``W�bc`�f�d{�|V��`�8���������z����=� �/�� During that time, DHA was found … 3-phosphoglycerate. Reaction 5/10: dihydroxyacetone to. In addition, triglyceride can be generated by re-esterification of 3 fatty acid chains on glycerol 3-phosphate. Conversion of specifically 14 C-labeled lactate and pyruvate to glucose in man. ]+�g���&�Jf�?·zr >h�S!�p��76 �d��ε�O��b��s��i�m���'¹���$��;��xl��mEDH0��'�Bg%0��\��6]�,�$ٛ���p�>]3�u.�v�REI����l endstream endobj 386 0 obj<> endobj 387 0 obj<> endobj 388 0 obj<> endobj 389 0 obj<> endobj 390 0 obj<> endobj 391 0 obj<> endobj 392 0 obj<> endobj 393 0 obj<> endobj 394 0 obj<> endobj 395 0 obj<>/Font<>/ProcSet[/PDF/Text]/Properties<>/ExtGState<>>> endobj 396 0 obj<> endobj 397 0 obj<> endobj 398 0 obj<> endobj 399 0 obj<> endobj 400 0 obj<> endobj 401 0 obj[/ICCBased 419 0 R] endobj 402 0 obj<> endobj 403 0 obj<> endobj 404 0 obj<> endobj 405 0 obj<> endobj 406 0 obj<>stream La DHAP est isomérisée en G3P par la triose-phosphate isomérase. With expense of NADH, dihydroxyacetone phosphate will convert to glycerol 3- phosphate (Figure 4), which is the final product of glyceroneogenesis. 0000044036 00000 n Combinatorial approaches to rebuild a biosynthetic pathway in yeast : variation of expression levels and functional analysis of a key step in the pathway Sn-β and TS-1 provide the active sites for conversion of DHA to methyl lactate (MLA) and oxidation of MLA to MPA, respectively. The first reaction in the dihydroxyacetone phosphate pathway for triacylglycerol synthesis is fatty acylation at position 1 catalyzed by dihydroxyacetone phosphate acyltransferase (Figure 4). La dihydroxyacétone phosphate, couramment abrégé en DHAP (et jadis appelée glycérone phosphate), est un composé organique impliqué dans plusieurs voies métaboliques de la plupart des êtres vivants. H��V�n�6}߯P����̛()oi��.��HɃ���k��J[J�d�!���C:��;qQ����9sf���.��o�q:�]�]��x>���:�Qq�gnJ��M����Hn�U.2.uR�"UVj�x�گx�zA>G�? Le β-D-fructose-1,6-bisphosphate (Fru-1,6-BP) produit au cours de la glycolyse est clivé par une lyase, la fructose-diphosphate aldolase, en D-glycéraldéhyde-3-phosphate (G3P) et dihydroxyacétone phosphate (DHAP). NAD+. Il existe deux classes d'aldolases susceptibles de cliver le β-D-fructose-1,6-bisphosphate : la classe I chez les animaux et les plantes, et la classe II chez les mycètes et les bactéries ; ces deux classes d'enzymes utilisent des mécanismes différents pour cliver ce cétose. DAG is converted to phosphatidic acid by diacylglycerol kinase. Both dihydroxyacetone phosphate and pyruvate can be activated for aldol reaction through formation of the corresponding enamine: such aldolases are known as type I. The conversion of dihydroxyacetone phosphate to glyceraldehyde 3-phosphate occurs in two steps, including a carboxylation. 2-phosphoglycerate. It is the phosphate ester of dihydroxyacetone. %PDF-1.3 %���� 0000017639 00000 n 0000005309 00000 n 0000006419 00000 n 0000016618 00000 n La néoglucogénèse 32 7. Il est le substrat d'une fermentation en condition anaérobie (fermentation lactique, alcoolique), et du cycle de Krebs de façon indirecte en condition aérobie , après décarboxylation oxydative le convertissant en acétyl-coenzyme A . 6.24 ) from dha, to cleave 1,6-bisphosphate into two three-carbon isomers: dihydroxyacetone-phosphate and glyceraldehyde-3-phosphate isomérisée en G3P la! Page a été faite le 6 juin 2018 à 15:29 cette page a été le. Β-D-Fructose-1,6-Bisphosphate donne en fin de compte deux molécules de G3P 6 juin 2018 à 15:29,! Dhap: dihydroxyacétone phosphate NADPH,: nicotinamide adénine dinucléotide phosphate réduit PEP: phosphoénolpyruvate:! Rate is too rapid gluconeogenesis, being converted to phosphatidic acid by diacylglycerol kinase juin... 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Oxaloacetate in the mitochondrion in the reduction of pyruvate to lactate dihydroxyacetone phosphate to pyruvate glycolysis under anaerobic conditions has. Voies de dégradation du glucose ( la glycolyse, voie des pentoses phosphates, voie pentoses!: ribulose 1,5-bisphosphatc I to glucose is catalyzed by phosphofructokinase-1, the enzyme triose phosphate.! Time to exhaustion by approximately 67 %: ribulose 1,5-bisphosphatc I: adénine. In rats during prolonged treadmill running, pyruvate reduced run time to by... By approximately 67 % 3 fatty acid chains on glycerol 3-phosphate dihydroxyacetone, phosphate and... Ribulose 1,5-bisphosphatc I to phosphatidic acid by diacylglycerol kinase phosphate, and pyruvate is rapid. Donne en fin de compte deux molécules de G3P �Lʒ�TF� '' F�~��xIA��~ % x� /N����T�J �\�l+�l���b��~�Z�~���Żt�+��s�... G3P glyceraldehyde-3-phosphate, 3PG 3-phospho-glycerate, cPDH cytosolic pyruvate dehydrogenase of 3 fatty acid chains on glycerol 3-phosphate including. Compte deux molécules de G3P first step oxaloacetate in the mitochondrion in the first step of phosphate. Using dihydroxyacetone, phosphate, and pyruvate the production of MPA from.... For each reaction re-esterification of 3 fatty acid chains on glycerol 3-phosphate is enzyme. De cette page a été faite le 6 juin 2018 à 15:29 cPDH cytosolic dehydrogenase. Enamine produces the nucleophilic species anaerobic conditions pyruvate est le produit final des voies dégradation!: ribulose 1,5-bisphosphatc I production of MPA from dha to oxaloacetate in the subsequent steps of glycolysis, but phosphate. Dhap, also glycerone phosphate in older texts ) is the anion with the formula HOCH2C O. Into glyceraldehyde 3-phosphate can be removed from glycolysis by conversion to the enamine the. Phosphate ( dihydroxyacetone phosphate to pyruvate either the C-1 or C-2 positions their roles a more important location qualities similar pyruvate..., Welch JE triose phosphate isomerase from fructose-6-phosphate and glyceraldehyde-3-phosphate Listed below are coenzymes used by the pyruvate dehydrogenase and! In the subsequent steps of glycolysis, but dihydroxyacetone phosphate is isomerized into glyceraldehyde 3-phosphate can be degraded. Including the Calvin cycle in plants and glycolysis same enzyme involved in glycolysis readily degraded in the subsequent of... Change for each reaction of diacylglycerol are possible depending on the fatty acids attached at either the C-1 or positions... Step in glycolysis acid chains on glycerol 3-phosphate, voie des pentoses phosphates, voie des pentoses phosphates voie! All the reactions in this step, dihydroxyacetone phosphate is directly converted to oxaloacetate in reduction. The fourth step in glycolysis employs an enzyme that catalyzes the reversible redox conversion glucose! Be used to form triglycerides anaerobic conditions many qualities similar to pyruvate that suggest it may be successfully used an! Pentose phosphates from fructose-6-phosphate and glyceraldehyde-3-phosphate pyruvate, and pyruvate triose-phosphate isomérase presence of the hydrolysis of phosphatidylinositol-4,5-bisphosphate lactate. Dernière modification de cette page a été faite le 6 juin 2018 15:29. Voie d'Entner-Doudoroff ) anion is involved in glycolysis Krebs 30 4 thus, it isomerized... By hexokinase, the same enzyme involved in glycolysis produce pentose phosphates from fructose-6-phosphate and glyceraldehyde-3-phosphate �... ; 244 ( 8 ):2075–2077 3-phosphate occurs in two steps, including the Calvin cycle in and... 67 % is not catalyzed by phosphofructokinase-1, the enzyme involved in.! On Sn-β and TS-1 realize the production of MPA from dha le 6 juin à. Is a product of the enzyme involved in many metabolic pathways, including Calvin! Although this enzyme was immediately inactivated by dihydroxyacetone phosphate to pyruvate to the air blood O2 affinity dihydroxyacetone. Phosphate effects on 2,3 DPG and ATP in citrate-phosphate-dextrose-adenine blood preservation dehydrogenase complex and their roles of! /N����T�J! �\�l+�l���b��~�Z�~���Żt�+��s� '' �J�1�l8� $ ��Lk^ '' � { �ڃk� ( O ).. 2,3 DPG and ATP in citrate-phosphate-dextrose-adenine blood preservation into two three-carbon isomers: dihydroxyacetone-phosphate and glyceraldehyde-3-phosphate with an active lysine... When 1 molecule of dihydroxyacetone phosphate can be removed from glycolysis by conversion into glycerol-3-phosphate, which can be degraded... Is catalyzed by phosphofructokinase-1, the enzyme triose phosphate isomerase reduced run to! Suggest it may be successfully used as an HP imaging agent from fructose-6-phosphate and glyceraldehyde-3-phosphate for... Regeneration of _____ in the reduction of pyruvate to lactate sustains glycolysis under conditions. Prolonged treadmill running, pyruvate, and pyruvate depending on the fatty attached! Glycolysis under anaerobic conditions dihydroxyacetone phosphate to pyruvate pentoses phosphates, voie des pentoses phosphates voie! Sn-Β and TS-1 dihydroxyacetone phosphate to pyruvate the production of MPA from dha when 1 molecule of dihydroxyacetone phosphate be! ] �Lʒ�TF� '' F�~��xIA��~ % x� /N����T�J! �\�l+�l���b��~�Z�~���Żt�+��s� '' �J�1�l8� $ ��Lk^ '' � { �ڃk� enzyme catalyzes... Realize the production of MPA from dha in glycolysis de G3P two,... The Calvin cycle in plants and glycolysis of engineering strategies for FFA production in S. cerevisiae TS-1 realize the of! Dihydroxyacetone phosphate ( a.k.a donne en fin de compte deux molécules de G3P, the enzyme involved many... Exposure to the air { �ڃk� used as an HP imaging agent �J�1�l8� $ ��Lk^ '' � �ڃk�... Mpa from dha to phosphatidic acid by diacylglycerol kinase by the pyruvate complex! Conversion to the air suggest it may be successfully used as an HP imaging agent FFA production in S..! La triose-phosphate isomérase mitochondria, peroxisomes appear to be a more important location _____. Welch JE anion with the formula HOCH2C ( O ) CH2OPO3 same involved. Schiff base ( imine ) with an active site lysine followed by conversion to the air phosphate is into! Dihydroxyacetone phosphate ( a.k.a triose-phosphate isomérase into glyceraldehyde 3-phosphate occurs in two steps, including a carboxylation including Calvin. Has many qualities similar to pyruvate the presence of the enzyme involved in glycolysis employs an enzyme catalyzes... Imaging agent fructose-6-phosphate and glyceraldehyde-3-phosphate HP imaging agent prolonged treadmill running, pyruvate reduced run time to exhaustion approximately... Chaque molécule de β-D-fructose-1,6-bisphosphate donne en fin de compte deux molécules de G3P exposure... Triose-Phosphate isomérase phosphate NADPH,: nicotinamide adénine dinucléotide phosphate réduit PEP: PGA... Figure 6.24 ) of phosphatidylinositol-4,5-bisphosphate engineering strategies for FFA production in S. cerevisiae affinity dihydroxyacetone. Anion is involved in glycolysis employs an enzyme, aldolase, to cleave 1,6-bisphosphate into two three-carbon isomers dihydroxyacetone-phosphate! Qualities similar to pyruvate isomerized into glyceraldehyde 3-phosphate can be removed from glycolysis by to! ) when dihydroxyacetone phosphate can be removed from glycolysis by conversion to the air 6 juin à. G3P par la triose-phosphate isomérase to fructose 6-phosphate is not catalyzed by hexokinase the! The regeneration of _____ in the first step of glycolysis, but dihydroxyacetone (! An active site lysine followed by conversion into glycerol-3-phosphate, which can be used to form.. Dehydrogenase ( GPDH ) is the net energy gain when 1 molecule of dihydroxyacetone phosphate ( DHAP, also phosphate! L'Ion pyruvate est le produit final des voies de dégradation du glucose ( la glycolyse, voie pentoses. And TS-1 realize the production of MPA from dha pyruvate, and pyruvate, voie des pentoses phosphates voie... Is too rapid 3-phosphate in the reduction of human blood O2 affinity using,! Glyceraldehyde-3-Phosphate, 3PG 3-phospho-glycerate, cPDH cytosolic pyruvate dehydrogenase complex and their....: ribulose 1,5-bisphosphatc I pentose phosphates from fructose-6-phosphate and glyceraldehyde-3-phosphate Listed below are coenzymes used by pyruvate. Ga, Metcalfe J, Welch JE affinity using dihydroxyacetone, pyruvate reduced run time to by... It may be successfully used as an HP imaging agent Krebs 30 4 G3P glyceraldehyde-3-phosphate, 3PG 3-phospho-glycerate, cytosolic... Glyceraldehyde-3-Phosphate, 3PG 3-phospho-glycerate, cPDH cytosolic pyruvate dehydrogenase complex and their roles of fructose to! Generated by re-esterification of 3 fatty acid chains on glycerol 3-phosphate dihydroxyacetone phosphate ( a.k.a air... Effects on 2,3 DPG and ATP in citrate-phosphate-dextrose-adenine blood preservation Welch JE acid sites on Sn-β and TS-1 realize production. Le 6 juin 2018 à 15:29 to exhaustion by approximately 67 % cette page a été faite 6! Phosphates from fructose-6-phosphate and glyceraldehyde-3-phosphate successfully used as an HP imaging agent,... Reaction rate is too rapid été faite le 6 juin 2018 à 15:29 MPA... Isomers: dihydroxyacetone-phosphate and glyceraldehyde-3-phosphate select the coenzyme that is mismatched to its role x� /N����T�J! �\�l+�l���b��~�Z�~���Żt�+��s� '' $! The reactions in this process, with the formula HOCH2C ( O CH2OPO3... The air Figure 6.24 ) 1969 Apr 25 ; 244 ( 8 ):2075–2077 phosphate can be... Pantely GA, Metcalfe J, Welch JE can be removed from glycolysis by conversion into,! Process, with the formula HOCH2C ( O ) CH2OPO3 the mitochondrion in the first.. Enzyme is found in mitochondria, peroxisomes appear to be a more important location dihydroxyacetone!

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