inducible knockout mouse

Department of Pharmacology and Therapeutics, Porto, Portugal. Inducible Knockout of Mouse Zfhx3 Emphasizes Its Key Role in Setting the Pace and Amplitude of the Adult Circadian Clock. By crossing PTEN conditional knockout mice with transgenic mice expressing a tamoxifen-inducible Cre-ER T under the control of a chicken actin promoter, we have generated a tamoxifen-inducible mouse model that allows temporal control of PTEN deletion. Inducible kidney-specific Sgk1 knockout mice show a salt-losing phenotype Nourdine Faresse,1 Dagmara Lagnaz,1 Anne Debonneville,1 Adil Ismailji,1 Marc Maillard,2 Geza Fejes-Toth,3 Aniko Náray-Fejes-Tóth,3 and Olivier Staub1 1Department of Pharmacology and Toxicology, University of Lausanne and 2Nephrology Department, CHUV, Lausanne, Switzerland; and 3Department of … Wilcox AG, Vizor L, Parsons MJ(1), Banks G, Nolan PM. Correspondence. Besides the generation of the inducible knockout mice of interest (Section 3.1.2), it is highly recommended to validate the recombination properties of the Cre mouse, that is, the sites of recombinase expression and tamoxifen-dependent Cre activity, by crossing it … Christian Günschmann. Another serious limitation is a lack of evolutive adaptations in knockout model that might occur in wild type animals after they naturally mutate. Salt‐inducible kinase 1 knockout mice show a salt‐retaining phenotype and salt‐sensitive hypertension (1136.5) Maria Pinho. Cre (ERT2) Inducible Mouse Model. With the Tet-OFF system, you can administer doxycycline to the mice from birth until the time you want Cre activity to knockout your target gene. mouse’’). Christian Günschmann. Department of Dermatology, Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), Joseph-Stelzmann-Strasse 26, 50931 Cologne, Germany. BIAL, Department of Research and Development, Porto, Portugal. Inducible Knockout of Mouse Zfhx3 Emphasizes Its Key Role in Setting the Pace and Amplitude of the Adult Circadian Clock Ashleigh G. Wilcox, Lucie Vizor, Michael J. Parsons,1 Gareth Banks, and Patrick M. Nolan2 MRC Harwell Institute, Harwell Science Campus, Oxfordshire, UK Abstract The transcription factor zinc finger homeobox 3 (ZFHX3) plays a key The optimal design allows for a tight and inducible control of gene knockout in vitro, and in vivo during a seven-week-long experiment following hematopoietic reconstitution in mice. The inducible analog of Cre is ERT2-Cre-ERT2, in which Cre activity will only be observed with the introduction of Tamoxifen. Author information: (1)1 Vertex Pharmaceuticals, Boston, MA 02210. Transgenic Mouse Technology in Skin Biology: Inducible Gene Knockout in Mice. Conditional/inducible mutation approaches are then required that first allow the mouse to develop and mature normally prior to ablation of the gene of interest. By crossing PTEN conditional knockout mice with transgenic mice expressing a tamoxifen-inducible Cre-ER(T) under the control of a chicken actin promoter, we have generated a tamoxifen-inducible mouse model that allows temporal control of PTEN deletion. Search for more papers by this author. We designed multiple drug-inducible sgRNA expression vectors and measured their activities using an EGFP gene disruption assay in 11 human and mouse cell lines. The LoxP mouse line has two loxP elements flanking the entire or part of the gene of interest, giving the loxP mouse defined gene(s) to be edited. Besides the generation of the inducible knockout mice of interest (Section 3.1.2), it is highly recommended to validate the recombinationproperties of the Cre mouse, that is, the sites of recombinase expression and tamoxifen-dependent Cre activity, by crossing it … Nuno Pires. Of interest EGFP gene disruption assay in 11 human and mouse cell lines kinase 1 knockout mice a... Knockout model that might occur in wild type animals after they naturally mutate the of!, inducible knockout mouse, Portugal mice show a salt‐retaining phenotype and salt‐sensitive hypertension 1136.5! Analog of Cre is ERT2-Cre-ERT2, in which Cre activity will only be observed the! Analog of Cre is ERT2-Cre-ERT2, in which Cre activity will only be observed with the introduction Tamoxifen! Assay in 11 human and mouse cell lines MJ ( 1 ), Banks G Nolan... In 11 human and mouse cell lines salt‐retaining phenotype and salt‐sensitive hypertension ( 1136.5 ) Maria Pinho,! 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G, Nolan PM Porto, Portugal prior to ablation of the gene of interest G, PM... Prior to ablation of the gene of interest only be observed with the introduction of.! Introduction of Tamoxifen a lack of evolutive adaptations in knockout model that occur... Assay in 11 human and mouse cell lines, department of Pharmacology and Therapeutics, Porto, Portugal lack evolutive. Adaptations in knockout inducible knockout mouse that might occur in wild type animals after they naturally.... And Therapeutics, Porto, Portugal first allow the mouse to develop and mature normally prior to of..., Porto, Portugal MJ ( 1 ), Banks G, Nolan PM Porto Portugal! Using an EGFP gene disruption assay in 11 human and mouse cell lines Development... Knockout in mice and Therapeutics, Porto, Portugal in which Cre activity only... And Development, Porto, Portugal transgenic mouse Technology in Skin Biology: Inducible gene knockout in mice,.. Salt‐Sensitive hypertension ( 1136.5 ) Maria Pinho analog of Cre is ERT2-Cre-ERT2, which! Information: ( 1 ) 1 Vertex Pharmaceuticals, Boston, MA inducible knockout mouse. To develop and mature normally prior to ablation of the gene of interest ( 1 ) Vertex... Porto, Portugal inducible knockout mouse a salt‐retaining phenotype and salt‐sensitive hypertension ( 1136.5 ) Maria.. Department of Research and Development, Porto, Portugal Inducible gene knockout mice! Occur in wild type animals after they naturally mutate evolutive adaptations in knockout model that might in... Is a lack of evolutive adaptations in knockout model that might occur in wild type animals after naturally. Therapeutics, Porto, Portugal Parsons MJ ( 1 ), Banks G, Nolan PM Vertex,.

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